Environmental radiation exposure at Chornobyl has not systematically affected the genomes or chemical mutagen tolerance phenotypes of local worms.

The 1986 disaster at the Chornobyl Nuclear Power Plant transformed the surrounding region into the most radioactive landscape known on the planet. Whether or not this sudden environmental shift selected for species, or even individuals within a species, that are naturally more resistant to mutagen exposure remains an open question. In this study, we collected, cultured, and cryopreserved 298 wild nematode isolates from areas varying in radioactivity within the Chornobyl Exclusion Zone. We sequenced and assembled genomes de novo for 20 Oscheius tipulae strains, analyzed their genomes for evidence of recent mutation acquisition in the field, and observed no evidence of an association between mutation and radioactivity at the sites of collection. Multigenerational exposure of each of these strains to several chemical mutagens in the lab revealed that strains vary heritably in tolerance to each mutagen, but mutagen tolerance cannot be predicted based on the radiation levels at collection sites, and Chornobyl isolates were not systematically more resistant than strains from undisturbed habitats. In sum, the absence of mutational signatures does not reflect unique capacity for tolerating DNA damage.

Environmental radiation exposure at Chornobyl has not systematically affected the genomes or mutagen tolerance phenotypes of local worms.

The 1986 disaster at the Chornobyl Nuclear Power Plant transformed the surrounding region into the most radioactive landscape known on the planet. Questions remain regarding whether this sudden environmental shift selected for species, or even individuals within a species, that are naturally more resistant to radiation exposure. We collected, cultured, and cryopreserved 298 wild nematodes isolates from areas varying in radioactivity within the Chornobyl Exclusion Zone. We sequenced and assembled genomes de novo for 20 Oschieus tipulae strains, analyzed their genomes for evidence of recent mutation acquisition in the field and saw no evidence of an association between mutation and radiation level at the sites of collection. Multigenerational exposure of each of these strains to several mutagens in the lab revealed that strains vary heritably in tolerance to each mutagen, but mutagen tolerance cannot be predicted based on the radiation levels at collection sites.

Transfer of 137Cs and 90Sr from soil-to-potato: Interpretation of the association from global fallout in Aomori to accidental release in Fukushima and Chornobyl.

Ceasium-137 and 90Sr are major artificial radionuclides that have been released into the environment. Soil-to-plant transfer of radionuclides is an important route to food contamination. The radionuclide activity concentrations in crops must be quantitatively predicted for estimating the internal radiation doses from food ingestion. In this study, soil and potato samples were collected from three study sites contaminated with different sources of 137Cs and 90Sr: Aomori Prefecture (global fallout) and two accidental release areas (Fukushima Prefecture and the Chornobyl exclusion zone). The 137Cs activity concentrations in the soil and potato samples widely ranged from 1.0 to 250,000 and from 0.048 to 200,000 Bq kg-1 dry weight, respectively. The soil-to-potato transfer factor of 137Cs also ranged widely (0.0015-1.1) and decreased with increasing concentration of exchangeable K. Meanwhile, the activity concentrations of 90Sr in the soil and potato samples were 0.50-64,000 and 0.027-18,000 Bq kg-1 dry weight respectively, and the soil-to-potato transfer factor of 90Sr was 0.023-0.74, decreasing with increasing concentration of exchangeable Ca. The specific activity ratios of 137Cs/Cs and 90Sr/Sr in the exchangeable fraction were similar to those in potatoes, with a factor of 3 in the ±95 % confidence intervals over six orders of magnitude and a factor of 2 in the ±95 % confidence intervals over five orders of magnitude, respectively. According to the data, the accuracy of predicting the activity concentrations of 137Cs and 90Sr in potatoes can be improved by applying the specific activity ratios of 137Cs/Cs and 90Sr/Sr in the exchangeable fraction. This approach accounts for variable factors such as the effects of K and Ca fertilization and soil characteristics. It also emphasizes the benefit of determining the stable Cs and Sr concentrations in potatoes and other crops prior to possible future contamination.

Uptake of Radionuclides by Bryophytes in the Chornobyl Exclusion Zone.

The "Chernobyl nuclear disaster" released huge amounts of radionuclides, which are still detectable in plants and sediments today. Bryophytes (mosses) are primitive land plants lacking roots and protective cuticles and therefore readily accumulate multiple contaminants, including metals and radionuclides. This study quantifies 137Cs and 241Am in moss samples from the cooling pond of the power plant, the surrounding woodland and the city of Prypiat. Activity concentrations of up to 297 Bq/g (137Cs) and 0.43 Bq/g (241Am) were found. 137Cs contents were significantly higher at the cooling pond, where 241Am was not detectable. Distance to the damaged reactor, amount of original fallout, presence of vascular tissue in the stem or taxonomy were of little importance. Mosses seem to absorb radionuclides rather indiscriminately, if available. More than 30 years after the disaster, 137Cs was washed out from the very top layer of the soil, where it is no more accessible for rootless mosses but possibly for higher plants. On the other hand, 137Cs still remains solved and accessible in the cooling pond. However, 241Am remained adsorbed to the topsoil, thus accessible to terrestrial mosses, but precipitated in the sapropel of the cooling pond.

The dogs of Chernobyl: Demographic insights into populations inhabiting the nuclear exclusion zone.

The 1986 Chernobyl nuclear disaster initiated a series of catastrophic events resulting in long-term and widespread environmental contamination. We characterize the genetic structure of 302 dogs representing three free-roaming dog populations living within the power plant itself, as well as those 15 to 45 kilometers from the disaster site. Genome-wide profiles from Chernobyl, purebred and free-breeding dogs, worldwide reveal that the individuals from the power plant and Chernobyl City are genetically distinct, with the former displaying increased intrapopulation genetic similarity and differentiation. Analysis of shared ancestral genome segments highlights differences in the extent and timing of western breed introgression. Kinship analysis reveals 15 families, with the largest spanning all collection sites within the radioactive exclusion zone, reflecting migration of dogs between the power plant and Chernobyl City. This study presents the first characterization of a domestic species in Chernobyl, establishing their importance for genetic studies into the effects of exposure to long-term, low-dose ionizing radiation.

Scots pine stands biomass assessment using 3D data from unmanned aerial vehicle imagery in the Chernobyl Exclusion Zone.

Thirty-five years after the accident, large forest areas in the Chernobyl Exclusion Zone still contain huge amounts of radionuclides released from the Chernobyl Nuclear Power Plant Unit 4 in April 1986. An assessment of the radiological and radioecological consequences of persistent radioactive contamination and development of remediation strategies for Chernobyl forests imply acquiring comprehensive data on their contamination levels and dynamics of biomass inventories. The most accurate forest inventory data can be obtained in ground timber cruises. However, such cruises in radioactive contaminated forest ecosystems in the Chernobyl Exclusion Zone result in radiation exposures of the personnel involved, which means the need for development of the remote sensing methods. The purpose of this study is to analyze the applicability and limitations of the photogrammetric method for the remote large-scale monitoring of aboveground biomass inventories. Based on field measurements, we estimated the biomass inventories in 31 Scots pine stands including both artificial plantations and natural populations. The stands differed significantly in age (from a few years in natural populations to 115 years in the oldest plantation), productivity (from 0.4 to 19.8 kg m-2), mean height (from 4.1 to 36 m), and other parameters. Photogrammetric data were obtained from the same stands using unmanned aerial vehicle (UAV). These data were then processed using two approaches to derive the canopy height model (CHM) parameters which were tested for correlation with the aboveground biomass inventories. In the first approach, we found that the inventories correlated well with the mean value of CHM of the site (R2 = 0.79). In the second approach, the total aboveground biomass was approximated by a function of the average height of trees detected at the site and the total crown projection area (R2 = 0.78). Among other local parameters, the total crown projection area was identified as the major factor impacting the accuracy of the aboveground biomass inventory estimates from the UAV survey data in both approaches. In the dense stands with the high total crown projections areas (more than 0.90), the average relative deviations of the UAV-based aboveground biomass estimates from the results of the field measurements were close to 0, which means the adequate accuracy of the UAV surveys data for radioecological monitoring purposes. The relative deviations of the UAV-based estimates in both approaches increased in the stands consisting of separated groups of trees, which indicates potential limitation of the approaches and need for their further development.

Determination of Characteristic vs Anomalous 135Cs/137Cs Isotopic Ratios in Radioactively Contaminated Environmental Samples.

A contamination with the ubiquitous radioactive fission product 137Cs cannot be assigned per se to its source. We used environmental samples with varying contamination levels from various parts of the world to establish their characteristic 135Cs/137Cs isotope ratios and thereby allow their distinction. The samples included biological materials from Chernobyl and Fukushima, historic ashed human lung tissue from the 1960s from Austria, and trinitite from the Trinity Test Site, USA. After chemical separation and gas reaction shifts inside a triple quadrupole ICP mass spectrometer, characteristic 135Cs/137Cs isotope signatures (all as per March 11, 2011) were obtained for Fukushima- (∼0.35) and Chernobyl-derived (∼0.50) contaminations, in agreement with the literature for these contamination sources. Both signatures clearly distinguish from the characteristic high ratio (1.9 ± 0.2) for nuclear-weapon-produced radiocesium found in human lung tissue. Trinitite samples exhibited an unexpected, anomalous pattern by displaying a low (<0.4) and nonuniform 135Cs/137Cs ratio. This exemplifies a 137Cs-rich fractionation of the plume in a nuclear explosion, where 137Cs is a predominant species in the fireball. The onset of 135Cs was delayed because of the longer half-life of its parent nuclide 135Xe, causing a spatial separation of gaseous 135Xe from condensed 137Cs, which is the reason for the atypical 135Cs/137Cs fractionation in the fallout at the test site.

Survival of the basidiomycete Schizophyllum commune in soil under hostile environmental conditions in the Chernobyl Exclusion Zone.

Radioactive contamination resulting from major nuclear accidents presents harsh environmental conditions. Inside the Chernobyl exclusion zone, even more than 30 years after the accident, the resulting contamination levels still does not allow land-use or human dwellings. To study the potential of basidiomycete fungi to survive the conditions, a field trial was set up 5 km south-south-west of the destroyed reactor unit. A model basidiomycete, the lignicolous fungus Schizophyllum commune, was inoculated and survival in the soil could be verified. Indeed, one year after inoculation, the fungus was still observed using DNA-dependent techniques. Growth led to spread at a high rate, with approximately 8 mm per day. This shows that also white-rot basidiomycetes can survive the harsh conditions in soil inside the Chernobyl exclusion zone. The unadapted fungal strain showed the ability to grow and thrive in the contaminated soil where both stress from radiation and heavy metals were present.